Identification of a protein linked to the genomic and subgenomic mRNAs of feline calicivirus and its role in translation

Herbert, Terence and Brierley, I. and Brown, T.D.K. (1997) Identification of a protein linked to the genomic and subgenomic mRNAs of feline calicivirus and its role in translation. Journal of General Virology, 78 (5). pp. 1033-1040. ISSN 0022-1317

Full content URL: http://jgv.microbiologyresearch.org/content/journa...

Full text not available from this repository.

Item Type:Article
Item Status:Live Archive

Abstract

125I protein labelling of oligo(dT)-selected RNA from feline calicivirus (FCV)-infected cells revealed that the genomic and 2.4 kb subgenomic RNAs of FCV are linked to a 15 kDa protein (VPg). Proteinase K treatment of FCV RNA, to remove VPg, led to a decrease in the translatability of the RNA, but there was no obvious change in the site of RNA initiation. Addition of the cap analogue 7-methylGTP to in vitro translations had no effect on the translation of FCV RNA, suggesting that FCV RNA is translated by a cap-independent mechanism. Further evidence that FCV RNA is translated by an unusual mechanism was obtained by translating FCV RNA in vitro at a range of K+ concentrations. FCV RNA was able to direct translation at K+ concentrations at which cellular RNA translation was inhibited.

Additional Information:cited By 91 The final published version of this article can be accessed online at http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-78-5-1033
Keywords:protein, genomic, subgenomic, mRNA
Subjects:B Subjects allied to Medicine > B200 Pharmacology, Toxicology and Pharmacy
Divisions:College of Science > School of Pharmacy
ID Code:28241
Deposited On:13 Sep 2018 13:44

Repository Staff Only: item control page