Molecular screening of blue mussels indicated high mid-summer prevalence of human genogroup II Noroviruses, including the pandemic “GII.4 2012” variants in UK coastal waters during 2013

Biswas, Subhajit and Jackson, Philippa and Shannon, Rebecca and Dulwich, Katherine and Sukla, Soumi and Dixon, Ronald A. (2017) Molecular screening of blue mussels indicated high mid-summer prevalence of human genogroup II Noroviruses, including the pandemic “GII.4 2012” variants in UK coastal waters during 2013. Brazilian Journal of Microbiology . ISSN 1517-8382

Documents
27761.pdf
[img]
[Download]
[img]
Preview
PDF
27761.pdf - Whole Document
Available under License Creative Commons Attribution 4.0 International.

544kB
Item Type:Article
Item Status:Live Archive

Abstract

Pandemic norovirus in coastal blue mussels during summer in UK
This molecular study is the first report, to the best of our knowledge, on identification of norovirus, NoV GII.4 Sydney 2012 variants, from blue mussels collected from UK coastal waters. Blue mussels (three pooled samples from twelve mussels) collected during the 2013 summer months from UK coastal sites were screened by RT-PCR assays. PCR products of RdRP gene for noroviruses were purified, sequenced and subjected to phylogenetic analysis. All the samples tested positive for NoVs. Sequencing revealed that the NoV partial RdRP gene sequences from two pooled samples clustered with the pandemic “GII.4 Sydney variants” whilst the other pooled sample clustered with the NoV GII.2 variants. This molecular study indicated mussel contamination with pathogenic NoVs even during mid-summer in UK coastal waters which posed potential risk of NoV outbreaks irrespective of season. As the detection of Sydney 2012 NoV from our preliminary study of natural coastal mussels interestingly corroborated with NoV outbreaks in nearby areas during the same period, it emphasizes the importance of environmental surveillance work for forecast of high risk zones of NoV outbreaks.

Keywords:Blue mussels, Norovirus, GII.4 Sydney variant, coastal water
Subjects:C Biological Sciences > C161 Marine Biology
Divisions:College of Science > School of Life Sciences
ID Code:27761
Deposited On:25 Jun 2017 20:28

Repository Staff Only: item control page