Single cell calcium imaging

Marsh, Katrina A. (1995) Single cell calcium imaging. In: Signal transduction protocols. Methods in Molecular Biology (41). Humana Press, pp. 229-238. ISBN 9781592595280

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Item Type:Book Section
Item Status:Live Archive

Abstract

As our understanding of the biological sciences expands, the boundaries between traditional disciplines tend to blur at the edges. Physiologists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and molecular biologists. However, the acquisition of new technologies can be a time-consuming and frustrating business, and unless an expert is on hand to give instruction, precious hours can be spent poring over half-described Methods sections with no guarantee of eventual success. The aim of Signal Transduction Protocols has been to get experts with "hands-on" experience in particular techniques to give detailed accounts of experimental protocols in a recipe-type format, which we hope will circumvent the problems of ambiguity often encountered when reading the literature. The techniques described in Signal Transduction Protocols are those that we think will be most useful in addressing questions in the area of receptor-mediated cell signaling, with particular regard to those receptors that are part of the G-protein-linked superfamily. To keep it to a manageable size, we have omitted any reference to electrophysiology and have instead concentrated on more biochemical approaches

Keywords:intracellular calcium, imaging, single cell
Subjects:C Biological Sciences > C700 Molecular Biology, Biophysics and Biochemistry
Divisions:College of Science > School of Life Sciences
ID Code:27576
Deposited On:24 May 2017 17:52

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