The effect of temperature on electrochemically driven denaturation monitored by SERS

Papadopoulou, Evanthia and Meneghello, Marta and Marafini, Pietro and Johnson, Robert P. and Brown, Tom and Bartlett, Philip N. (2015) The effect of temperature on electrochemically driven denaturation monitored by SERS. Bioelectrochemistry, 106 . pp. 353-358. ISSN 1567-5394

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Item Type:Article
Item Status:Live Archive

Abstract

Scanning the electrochemical potential negative results in the gradual denaturation of dsDNA immobilised at a nanostructure gold electrode, the DNA melting is monitored by SERS. We demonstrate the effect of the experimental temperature on the electrochemically driven melting (E-melting) by carrying out experiments between 10 and 28 °C using two DNA duplexes (20 and 21 base pairs). Significant temperature dependence for both the melting potentials, Em, and the steepness of the melting curves was found over the range 10 to 18 °C. Above 18 °C the results were found to be independent of temperature. The relative temperature insensitivity of the melting potentials above 18 °C is advantageous for the application of the electrochemically driven melting technique because precise temperature control is not necessary for measurements that are carried out around room temperature. Conversely temperature dependence below 18 °C offers a way to improve discrimination for highly similar DNA sequences.

Keywords:SERS, Electrochemical Melting, DNA, Raman Spectroscopy, NotOAChecked
Subjects:F Physical Sciences > F170 Physical Chemistry
Divisions:College of Science > School of Chemistry
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ID Code:27416
Deposited On:02 May 2017 19:37

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