Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines

Najlah, M. and Kadam, A. and Wan, K.-W. and Ahmed, W. and Taylor, K. M. G. and Elhissi, A. M. A. (2016) Novel paclitaxel formulations solubilized by parenteral nutrition nanoemulsions for application against glioma cell lines. International Journal of Pharmaceutics, 506 (1-2). pp. 102-109. ISSN 0378-5173

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Abstract

The aim of this study is to investigate using nanoemulsion formulations as drug-delivery vehicles of paclitaxel (PX), a poor water-soluble anticancer drug. Two commercially available nanoemulsion fat formulations (Clinoleic 20 and Intralipid 20) were loaded with PX and characterised based on their size, zeta potential, pH and loading efficiency. The effect of formulation on the cytotoxicity of PX was also evaluated using MTT assay. The droplet size of the Clinoleic emulsion increased from 254.1 nm to 264.7 nm when paclitaxel (6 mg/ml) was loaded into the formulation, compared to the drug-free formulation. Similarly, the droplet size of Intralipid increased from 283.3 to 294.6 nm on inclusion of 6 mg/ml paclitaxel. The Polydispersity Indexes (PDIs) of all the nanoemulsion formulations (Clinoleic and Intralipid) were less than 0.2 irrespective of paclitaxel concentration indicating that all nanoemulsion formulations used were homogeneously sized. The pH range for the Clinoleic formulations (7.1-7.5) was slightly higher than that of the Intralipid formulations (6.5-6.9). The zeta potential of linoleic had a greater negative value than that of Intralipid. Loading efficiencies for paclitaxel were 70.4-80.2 and 44.2-57.4 for Clinoleic and Intralipid formulations, respectively. Clinoleic loaded with paclitaxel decreased the viability of U87-MG cell to 6.4 ± 2.3, compared to Intralipid loaded with paclitaxel (21.29 ± 3.82). Both nanoemulsions were less toxic to the normal glial cells (SVG-P12), decreasing the cell viability to 25-35. This study suggests that nanoemulsions are useful and potentially applicable vehicles of paclitaxel for treatment of glioma. © 2016 Elsevier B.V. All rights reserved.

Keywords:antineoplastic agent, clinoleic, drug carrier, emulsion, excipient, nanoparticle, paclitaxel, phospholipid, soybean oil, soybean oil, phospholipid emulsion, vegetable oil, administration and dosage, chemistry, drug delivery system, glioma, human, medicinal chemistry, parenteral nutrition, particle size, procedures, solubility, tumor cell line, Antineoplastic Agents, Cell Line, Tumor, Chemistry, Pharmaceutical, Drug Carriers, Drug Delivery Systems, Emulsions, Excipients, Humans, Nanoparticles, Phospholipids, Plant Oils, NotOAChecked
Subjects:F Physical Sciences > F200 Materials Science
B Subjects allied to Medicine > B100 Anatomy, Physiology and Pathology
J Technologies > J510 Materials Technology
Divisions:College of Science > School of Mathematics and Physics
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ID Code:27095
Deposited On:03 May 2017 15:00

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