Simplifying the delivery of melanocytes and keratinocytes for the treatment of vitiligo using a chemically defined carrier dressing

Eves, Paula and Bullet, Nial A. and Haddow, David and Beck, Alison J. and Layton, Christopher and Way, Louise and Shard, Alex G. and Gawkrodger, David J. and MacNeil, Sheila (2008) Simplifying the delivery of melanocytes and keratinocytes for the treatment of vitiligo using a chemically defined carrier dressing. Journal of Investigative Dermatology, 128 (6). pp. 1554-1564. ISSN 0022-202X

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Abstract

Obtaining pigmentary function in autologous skin grafts is a current challenge for burn surgeons as is developing reliable robust grafting strategies for patients with vitiligo and piebaldism. In this paper, we present the development of a simple methodology for delivering cultured keratinocytes and melanocytes to the patient that is of low risk for the patient but also user friendly for the surgeon. In this study, we examined the ability of keratinocytes and melanocytes to transfer from potential cell carriers under different media conditions to an in vitro human wound bed model. The number of melanocytes transferred, their location within the neoepidermis, and their ability to pigment were evaluated as preclinical end points. Two inert substrates (polyvinyl chloride and silicone sheets) and three candidate plasma-polymerized coatings with controlled surface chemistry deposited on these substrates were explored. Two media for expansion of cells, Greens, currently used clinically (but which contains fetal calf serum), and a serum-free alternative, M2 (melanocyte medium), were explored. Reproducible transfer of physiologically relevant numbers of melanocytes capable of pigmentation from the coculture of melanocytes and keratinocytes was obtained using either Greens medium or M2 medium, and a silicone carrier pretreated with 20% carboxylic acid deposited by plasma polymerization. © 2008 The Society for Investigative Dermatology.

Keywords:Bandages, Cell adhesion, Coculture techniques, Culture techniques, Epidermis, Humans, Keratinocytes, Melanocytes, Models, Polyvinyl chloride, Silicones, Skin transplantation, skin, Tissue engineering, Vitiligo
Subjects:C Biological Sciences > C131 Applied Cell Biology
A Medicine and Dentistry > A100 Pre-clinical Medicine
H Engineering > H673 Bioengineering
Divisions:Professional services
ID Code:26986
Deposited On:21 Apr 2017 08:10

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