Observation of endotoxins neutralization by polymyxin with light microscope

Dixon, Ron and Hing, H L and Sahalan, A (2009) Observation of endotoxins neutralization by polymyxin with light microscope. Annals of Microscopy , 9 . pp. 88-92. ISSN UNSPECIFIED

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Abstract

The outer membrane component of Gram negative bacteria are composed of lipopolysaccharides
(LPS). LPS are endotoxins which often caused the fatal condition known as septic
shock. However, a peptide antibiotic known as polymyxin B (PMB) was detected to neutralize these
endotoxin molecules. However, due to its toxicity, PMB remains unused as a treatment for endotoxic
shock. But it has been accepted widely as a model substance in studying LPS neutralization. At
present, method of observing LPS alone or LPS interaction in the presence of PMB is carried out
with an electron micrograph. This laborious method requires highly skilled technicians to conduct
simple experiment. However, a simplifi ed and rapid method was introduced by using the light
microscope. LPS staining was carried out with Sudan black. As a result a relatively good image
for observation was obtained. The LPS was seen as dark ribbon like strand forming loops. Upon the exposure to PMB, the LPS strands were broken down into small fragmented molecules.
This method has given a simple, low cost and quick observation technique. It also provides a good platform for screening many new compounds that neutralizes LPS.

Item Type:Article
Additional Information:The outer membrane component of Gram negative bacteria are composed of lipopolysaccharides (LPS). LPS are endotoxins which often caused the fatal condition known as septic shock. However, a peptide antibiotic known as polymyxin B (PMB) was detected to neutralize these endotoxin molecules. However, due to its toxicity, PMB remains unused as a treatment for endotoxic shock. But it has been accepted widely as a model substance in studying LPS neutralization. At present, method of observing LPS alone or LPS interaction in the presence of PMB is carried out with an electron micrograph. This laborious method requires highly skilled technicians to conduct simple experiment. However, a simplifi ed and rapid method was introduced by using the light microscope. LPS staining was carried out with Sudan black. As a result a relatively good image for observation was obtained. The LPS was seen as dark ribbon like strand forming loops. Upon the exposure to PMB, the LPS strands were broken down into small fragmented molecules. This method has given a simple, low cost and quick observation technique. It also provides a good platform for screening many new compounds that neutralizes LPS.
Keywords:endotoxins, polymyxin B, microscope
Subjects:C Biological Sciences > C521 Medical Microbiology
C Biological Sciences > C500 Microbiology
Divisions:College of Science > School of Life Sciences
ID Code:2661
Deposited By: Ron Dixon
Deposited On:11 Jun 2010 09:15
Last Modified:22 Aug 2012 09:09

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