Obesity and sex influence insulin resistance and total and multimer adiponectin levels in adult neutered domestic shorthair client-owned cats

Bjornvad, C. R. and Rand, J. S. and Tan, H. Y. and Jensen, K. S. and Rose, F. J. and Armstrong, P. J. and Whitehead, J. P. (2014) Obesity and sex influence insulin resistance and total and multimer adiponectin levels in adult neutered domestic shorthair client-owned cats. Domestic Animal Endocrinology, 47 (1). pp. 55-64. ISSN 0739-7240

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Abstract

In this study, we estimated insulin sensitivity and determined plasma concentrations of total-, low-molecular-weight (LMW), and high-molecular-weight (HMW) adiponectin and leptin in 72 domestic shorthair, neutered, client-owned cats. Glucose tolerance was assessed with an intravenous glucose tolerance test and body fat percentage (BF) was measured with dual-energy x-ray absorptiometry. Total adiponectin was measured with 2 different ELISAs. Low-molecular-weight and HMW adiponectin plasma concentrations were determined by Western blot analysis after sucrose-gradient velocity centrifugation, and the adiponectin multimer ratio SA = HMW/(HMW + LMW) was calculated. Differences in glucose tolerance, leptin, total adiponectin, and multimer ratio among lean (BF% <35; n = 26), overweight (35 <BF% <45; n = 28), and obese (BF% >45; n = 18) cats as well as between male (n = 34) and female (n = 38) neutered cats were evaluated by linear regression and 2-way ANOVA. Sex and age were included as covariates for analysis of BF%, whereas BF%, fat mass, and lean body mass were covariates for analysis of sex differences. Increased BF% was negatively correlated with multimer ratio (SA, r = -45; P < 0.002), whereas no differences were found in total adiponectin concentrations among BF% groups (P > 0.01). Male cats had indices of decreased insulin tolerance and significantly lower total adiponectin concentrations than did female cats (mean ± SEM, 3.7 ± 0.4 vs 5.4 ± 0.5 μg/mL; P < 0.02). Altered SAs could contribute to an obesity-associated decreasing glucose tolerance in cats, and low total adiponectin concentrations may relate to increased risk of diabetes mellitus in neutered male cats. © 2014 Elsevier Inc.

Keywords:Felidae, adipocytokine, adiponectin, animal, animal disease, article, blood, cat, cat disease, chemistry, dual-energy x-ray absorptiometry, Feline, female, gender, genetics, insulin resistance, male, metabolism, obesity, physiology, sex difference, Adipokine, Gender, Adiponectin, Animals, Cat Diseases, Cats, Sex Factors
Subjects:D Veterinary Sciences, Agriculture and related subjects > D322 Animal Physiology
D Veterinary Sciences, Agriculture and related subjects > D100 Pre-clinical Veterinary Medicine
Divisions:College of Science > School of Life Sciences
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ID Code:26211
Deposited On:14 Mar 2018 16:47

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