Characterization of cold-induced remodelling reveals depot-specific differences across and within brown and white adipose tissues in mice

Jia, R. and Luo, X.-Q. and Lin, C.-X. and Qiao, H. and Wang, N. and Yao, T. and Barclay, J. L. and Whitehead, J. P. and Luo, X. and Yan, J.-Q. (2016) Characterization of cold-induced remodelling reveals depot-specific differences across and within brown and white adipose tissues in mice. Acta Physiologica, 217 (4). pp. 311-324. ISSN 1748-1708

Full content URL: https://doi.org/10.1111/apha.12688

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Abstract

Brown and beige adipose tissues dissipate energy in the form of heat via mitochondrial uncoupling protein 1, defending against hypothermia and potentially obesity. The latter has prompted renewed interest in understanding the processes involved in browning to realize the potential therapeutic benefits. To characterize the temporal profile of cold-induced changes and browning of brown and white adipose tissues in mice. Methods: Male C57BL/6J mice were singly housed in conventional cages under cold exposure (4°C) for 1, 2, 3, 4, 5 and 7days. Food intake and body weight were measured daily. Interscapular brown adipose tissue (iBAT), inguinal subcutaneous (sWAT) and epididymal white adipose tissue (eWAT) were harvested for histological, immunohistochemical, gene and protein expression analysis. Results: Upon cold exposure, food intake increased, whilst body weight and adipocyte size were found to be transiently reduced. iBAT mass was found to be increased, whilst sWAT and eWAT were found to be transiently decreased. A combination of morphological, genetic (Ucp-1, Pgc-1α and Elov13) and biochemical (UCP-1, PPARγ and aP2) analyses demonstrated the depot-specific remodelling in response to cold exposure. Conclusion: Our results demonstrate the differential responses to cold-induced changes across discrete BAT and WAT depots and support the notion that the effects of short-term cold exposure are achieved by expansion, activation and increasing thermogenic capacity of iBAT, as well as browning of sWAT and, to a lesser extent, eWAT. © 2016 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd

Keywords:cyclophilin, Elov13 protein, glucose transporter 1, glucose transporter 4, messenger RNA, peroxisome proliferator activated receptor gamma, peroxisome proliferator activated receptor gamma coactivator 1alpha, protein, unclassified drug, uncoupling protein 1, adipocyte, animal experiment, animal tissue, Article, body weight, brown adipose tissue, cold exposure, food intake, histology, interscapular brown adipose tissue, lipid composition, male, mitochondrial biogenesis, mouse, nonhuman, polyacrylamide gel electrophoresis, priority journal, reverse transcription polymerase chain reaction, subcutaneous drug administration, Western blotting, white adipose tissue
Subjects:C Biological Sciences > C130 Cell Biology
B Subjects allied to Medicine > B120 Physiology
Divisions:College of Science > School of Life Sciences
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ID Code:26198
Deposited On:05 Mar 2018 15:44

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