Size-dependent cellular uptake of exosomes

Caponnetto, Federica and Manini, Ivana and Skrap, Miran and Palmai-Pallag, Timea and Di Loreto, Carla and Beltrami, Antonio Paolo and Cesselli, Daniela and Ferrari, Enrico (2017) Size-dependent cellular uptake of exosomes. Nanomedicine: Nanotechnology, Biology and Medicine, 13 (3). ISSN 1549-9634

Full content URL: http://www.nanomedjournal.com/article/S1549-9634(1...

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Item Type:Article
Item Status:Live Archive

Abstract

The ability of exosomes to elicit specific cellular responses suggests that they may be increasingly used as therapeutics. Their vesicular nature makes them suitable as potential nanocarriers for drugs or nucleic acids delivery. Here we address the question whether the method of preparation of enriched exosomal fractions can affect their uptake by cells and their ability to trigger a response. We compared ultracentrifugation and polymer-based precipitation methods on supernatants of glioma-associated stem cells isolated from a high-grade glioma patient. We determined particle size distributions after purification and their correlation with uptake, proliferation and migration in glioblastoma cell cultures. Our findings indicate that polymer-based precipitation leads to smaller particle size distributions, faster uptake by target cells and increased cellular motility. The different effect that isolation method-dependent populations of particles have on cell motility suggests their size distribution could also profoundly affect exosome therapeutic potential.

Keywords:AFM, Cellular uptake, Exosomes, Flow cytometry, GASC, Glioma
Subjects:C Biological Sciences > C760 Biomolecular Science
C Biological Sciences > C560 Biotechnology
C Biological Sciences > C130 Cell Biology
Divisions:College of Science > School of Life Sciences
ID Code:25547
Deposited On:06 Jan 2017 11:11

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