An assessment of the antibacterial activity in larval excretion/secretion of four species of insects recorded in association with corpses, using Lucilia sericata Meigen as the marker species

Barnes, K. M. and Gennard, D. E. and Dixon, R. A. (2010) An assessment of the antibacterial activity in larval excretion/secretion of four species of insects recorded in association with corpses, using Lucilia sericata Meigen as the marker species. Bulletin of Entomological Research, 100 (6). pp. 635-640. ISSN 0007-4853

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Abstract

The relative antibacterial activities of excretion/secretion (ES) from two carrion-feeding
insects, Calliphora vicina Robineau-Desvoidy and Dermestes maculatus
DeGeer, and a detritivore, Tenebrio molitor Linnaeus, were compared to that of
Lucilia sericata Meigen, a species with ES of known antibacterial capacity, in order
to explore the antimicrobial potential of other carrion and detritivore species.
Viable counts were used to assess time-kill of ES against five bacterial species,
Staphylococcus aureus, Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa and
Proteus mirabilis. Antibacterial activity was recorded in all four insect species
although T. molitor and D. maculatus were the most effective in controlling growth
of P. mirabilis. The blowflies were more effective in controlling a wider range of
both Gram-positive and Gram-negative bacteria. The larval ES from all species was
shown to reduce bacterial growth rate although differences in antibacterial
spectrum were noted and the degree of potency varied between the four species.
These differences may be explained ecologically by the different colonisation times
of each insect species on the corpse. Overall, this study demonstrates that research
into other carrion-feeding insect species has potential to provide an increased
source of antimicrobial chemicals to broaden the range of bacterial species beyond
that currently controlled using L. sericata.

Item Type:Article
Additional Information:The relative antibacterial activities of excretion/secretion (ES) from two carrion-feeding insects, Calliphora vicina Robineau-Desvoidy and Dermestes maculatus DeGeer, and a detritivore, Tenebrio molitor Linnaeus, were compared to that of Lucilia sericata Meigen, a species with ES of known antibacterial capacity, in order to explore the antimicrobial potential of other carrion and detritivore species. Viable counts were used to assess time-kill of ES against five bacterial species, Staphylococcus aureus, Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa and Proteus mirabilis. Antibacterial activity was recorded in all four insect species although T. molitor and D. maculatus were the most effective in controlling growth of P. mirabilis. The blowflies were more effective in controlling a wider range of both Gram-positive and Gram-negative bacteria. The larval ES from all species was shown to reduce bacterial growth rate although differences in antibacterial spectrum were noted and the degree of potency varied between the four species. These differences may be explained ecologically by the different colonisation times of each insect species on the corpse. Overall, this study demonstrates that research into other carrion-feeding insect species has potential to provide an increased source of antimicrobial chemicals to broaden the range of bacterial species beyond that currently controlled using L. sericata.
Keywords:Lucilia sericata, Calliphora vicina, Tenebrio molitor, Dermestes maculatus
Subjects:D Veterinary Sciences, Agriculture and related subjects > D320 Animal Health
F Physical Sciences > F410 Forensic Science
C Biological Sciences > C340 Entomology
Divisions:College of Science > School of Life Sciences
ID Code:2515
Deposited By: Dorothy Gennard
Deposited On:21 May 2010 09:05
Last Modified:13 Mar 2013 08:38

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