The effect of acute bovine colostrum supplementation on neutrophil responses to prolonged cycling

Jones, A. and Thatcher, R. and Davison, G. (2011) The effect of acute bovine colostrum supplementation on neutrophil responses to prolonged cycling. In: 10th Symposium of the International Society of Exercise and Immunology, 11 - 13 July 2011, Oxford.

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Abstract

Bovine colostrum (BC) supplementation for periods of 4-12 weeks has been shown to reduce the magnitude of, or speed recovery from, exercise-induced immunodepression (1). The purpose of this study was to identify whether acute BC supplementation prior to a bout of prolonged exercise has any effect on neutrophil function. Seven healthy males (age: 23.3 ± 3.9 years; mean ± SD) participated in 2 main trials in a randomised order. Subjects consumed either BC or placebo 1 hour prior to 2.5 hours of cycling at approximately 55% VO2 max (30 g), immediately prior (5 g) and midway through the exercise (5 g). Venous blood samples were obtained prior to consumption of the supplement (BAS), 1 hour post-drink (immediately pre-exercise: PRE), immediately post- exercise (POST) and 1 hour post-exercise (1-POST). Neutrophil counts were measured using an automated haematology analyser. In-vitro stimulated neutrophil oxidative burst responses (OBA) to PMA and fMLP were measured by chemiluminescence (CL) assay and expressed per neutrophil. Repeated measures 1 way ANOVA and post hoc paired t-tests (Bonferroni corrected) revealed significant increases at both post-exercise timepoints for blood neutrophil count (P < 0.01). For fMLP-stimulated OBA, 2-way repeated measures ANOVA revealed a main effect of time (P < 0.001) and a trend for a main effect of trial (P = 0.068) but no time × trial interaction (P > 0.05). For PMA-stimulated OBA, there was a main effect of time (P = 0.01) but no main effect of trial or time × trial interaction (P > 0.05). Post hoc comparisons demonstrated significant decreases below BAS at POST (P < 0.001) for PMA-stimulated OBA and at POST and 1-POST for fMLP-stimulated OBA (P < 0.05). These results suggest that fMLP-stimulated OBA is generally higher with acute BC supplementation but the overall temporal pattern (a post-exercise decrease) is similar between trials. These preliminary results show trends to support the idea that BC may enhance neutrophil functions by a direct and immediate mechanism, in agreement with findings from in-vitro studies (2). At present statistical power is low and the intention is to increase the sample size to 12.

1. Davison & Diment. (2010) Br J Nutr, 103, 1425-1432.
2. Sugisawa et al. (2001). Biol Neonate, 79, 140-144.

Keywords:endurance exercise, innate immunity, Nutritional supplements
Subjects:C Biological Sciences > C550 Immunology
C Biological Sciences > C600 Sports Science
B Subjects allied to Medicine > B400 Nutrition
B Subjects allied to Medicine > B120 Physiology
Divisions:College of Social Science > Lincoln Institute of Health
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ID Code:23137
Deposited On:14 May 2016 19:10

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