The biocompatibility of apoferritin-encapsulated PbS quantum dots

Turyanska, L. and Bradshaw, T. D. and Sharpe, J. and Li, M. and Mann, S. and Thomas, N. R. and Patanè, A. (2009) The biocompatibility of apoferritin-encapsulated PbS quantum dots. Small, 5 (15). pp. 1738-1741. ISSN 1613-6810

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Abstract

The biocompatibility of a nanocomposite based on a Pbs quantum dots (QD) entrapped in the hollow core of an apoferritin protein cage is examined. This offers a water-soluble hybrid construct with stable and tunable fluorescence emission at wavelengths larger than 1000nm. Native polyacrylamide gel-electrophoresis studies show that the PbS QDs do not alter the external surface of apoferritin or its migratory behavior. Apoferritin is obtained from ferritin molecules by reductive dissolution of its iron oxide core. MTT assays were performed on normal and cancer cell lines and recorded the cell viability after treatment with AFt-PbS at different concentrations and up to 72-hour. Cells were cultured under optimum conditions in RPMI nutrient medium supplemented with 10 fetal bovine serum, and subcultivated twice weekly to maintain logarithmic growth. Cells were seeded into 96-well microtiter plates at a density of 5 � 103 per well and allowed 24-hour to adhere before AFt, PbS QDs and AFt-PbS composites were introduced with final concentrations of QDs.

Keywords:96-Well microtiter plate, A-density, After-treatment, Apoferritin, At-wavelength, Cancer cell lines, Cell viability, External surfaces, Ferritin molecules, Fetal bovine serum, Fluorescence emission, Hollow cores, Logarithmic growth, MTT assays, Nutrient medium, Optimum conditions, PbS, Polyacrylamide gels, Protein cages, Quantum Dot, Quantum dots, Reductive dissolution, Biocompatibility, Cell culture, Cell membranes, Cytotoxicity, Dissolution, Electrophoresis, Gelation, Iron oxides, Photoluminescence, Semiconducting lead compounds, Semiconductor quantum dots, Bovinae, apoferritin, biomaterial, lead, lead sulfide, nanocomposite, sulfide, article, human, luminescence, polyacrylamide gel electrophoresis, tumor cell line, ultrastructure, Apoferritins, Biocompatible Materials, Cell Line, Tumor, Electrophoresis, Polyacrylamide Gel, Humans, Nanocomposites, Sulfides
Subjects:B Subjects allied to Medicine > B230 Pharmacy
F Physical Sciences > F300 Physics
F Physical Sciences > F100 Chemistry
Divisions:College of Science > School of Chemistry
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ID Code:22527
Deposited On:25 Mar 2016 21:31

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