An increased expression of the mGlu5 receptor protein following LTP induction at the perforant path-dentate gyrus synapse in freely moving rats

Manahan-Vaughan, D. and Ngomba, Richard T. and Storto, M. and Kulla, A. and Catania, M. V. and Chiechio, S. and Rampello, L. and Passarelli, F. and Capece, A. and Reymann, K. G. and Nicoletti, F. (2003) An increased expression of the mGlu5 receptor protein following LTP induction at the perforant path-dentate gyrus synapse in freely moving rats. Neuropharmacology, 44 (1). pp. 17-25. ISSN 0028-3908

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Abstract

The involvement of metabotropic glutamate (mGlu) receptors in the induction of long-term potentiation (LTP) in vivo has been consistently documented. We have investigated whether LTP induction in the dentate gyrus of rats leads to changes in expression of mGlu2/3 or -5 receptor subtypes in the hippocampus. LTP was induced at the medial perforant path–dentate gyrus synapses, and mGlu receptor expression was examined by Western blot or in situ hybridization. An up-regulation of mGlu5 receptors was observed in the hippocampus both 24 and 48 h following LTP induction. This effect was restricted to the dentate gyrus and CA1 region, whereas no changes in mGlu5 receptor protein (but an increase in mRNA levels) were observed in the CA3 region. The increased expression of mGlu5 receptors was directly related to the induction of LTP, because it was not observed when tetanic stimulation was carried out in animals treated with the NMDA receptor antagonist, 2-amino-5-phosphonopentanoate (AP5). Western blot analysis also showed a reduced expression of mGlu2/3 receptors in the whole hippocampus 24 h after LTP induction, indicating that the increased expression of mGlu5 receptors was specific. These data suggest that an up-regulation of mGlu5 receptors is a component of the plastic changes that follow the induction of LTP at the perforant path–dentate gyrus synapse.

Keywords:2 amino 5 phosphonovaleric acid, messenger RNA, metabotropic receptor, metabotropic receptor 2, metabotropic receptor 3, metabotropic receptor 5, n methyl dextro aspartic acid receptor stimulating agent, pentobarbital, receptor protein, receptor subtype, unclassified drug, animal experiment, animal tissue, article, brain depth stimulation, controlled study, dentate gyrus, hippocampus, in situ hybridization, long term potentiation, male, nerve cell plasticity, nonhuman, perforant nerve tract, priority journal, protein expression, rat, receptor upregulation, synapse, Western blotting, 2-Amino-5-phosphonovalerate, Animals, Blotting, Western, Electroencephalography, Evoked Potentials, Injections, Intraventricular, Long-Term Potentiation, Perforant Pathway, Rats, Rats, Wistar, Receptors, Metabotropic Glutamate, RNA, Messenger, Synapses, Synaptic Transmission
Subjects:B Subjects allied to Medicine > B210 Pharmacology
B Subjects allied to Medicine > B140 Neuroscience
Divisions:College of Science > School of Pharmacy
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ID Code:22169
Deposited On:24 Feb 2016 14:34

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