Neuroprotective effect of prenyloxycoumarins from edible vegetables

Epifano, F. and Molinaro, G. and Genovese, S. and Ngomba, Richard T. and Nicoletti, F. and Curini, M. (2008) Neuroprotective effect of prenyloxycoumarins from edible vegetables. Neuroscience Letters, 443 (2). pp. 57-60. ISSN 0304-3940

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Abstract

The present study is designed to investigate the effect of some natural prenyloxyphenylpropanoids as neuroprotective agents against NMDA-induced toxicity in mixed cortical cell cultures containing both neurons and astrocytes. Excitotoxicity was induced by exposure of cultures to NMDA (100 μM ) at room temperature in a HEPES-buffered salt solution followed by incubation at 37 °C for the following 24 h in MEM-Eagle's supplemented with 15.8 mM NaHCO3 and 25 mM glucose. Tested compounds were mixed with NMDA. Neuronal injury was measured in all experiments by examination of cultures with phase-contrast microscopy at 20x, 18-20 h after the insult while neuronal damage was quantitatively assessed by counting dead neurons stained with trypan blue and by measuring lactate dehydrogenase (LDH) released in the medium. Results showed that only natural prenyloxyphenylpropanoids containing a coumarin nucleus, namely 7-isopentenyloxycoumarin and auraptene, both found in nature from plants belonging to the genus Citrus and other of the family of Rutaceae, including edible ones, exerted a good dose-dependent manner protective effect against NMDA-induced neurotoxicity in particular at concentrations ranging from 1 to 10 μM . © 2008 Elsevier Ireland Ltd. All rights reserved.

Keywords:7 isopentenyloxycoumarin, auraptene, coumarin derivative, lactate dehydrogenase, n methyl dextro aspartic acid, prenyloxyphenylpropanoid derivative, trypan blue, animal cell, article, brain cell, cell culture, citrus fruit, controlled study, excitotoxicity, fetus, mouse, neuroprotection, neurotoxicity, nonhuman, phase contrast microscopy, priority journal, Rutaceae, vegetable, Animals, Astrocytes, Cells, Cultured, Coumarins, Excitatory Amino Acid Agonists, Mice, N-Methylaspartate, Nerve Degeneration, Neurons, Neuroprotective Agents, Vegetables
Subjects:B Subjects allied to Medicine > B230 Pharmacy
B Subjects allied to Medicine > B140 Neuroscience
Divisions:College of Science > School of Pharmacy
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ID Code:22157
Deposited On:12 Feb 2016 12:09

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