Gonzalez-Rodriguez, Jose and Perez Juan, Pedro and Luque de Castro, Maria Dolores (2002) Flow injection determination of total catechins and procyanidins in white and red wines. Innovative Food Science and Emerging Technologies, 3 (3). pp. 289-293. ISSN 1466-8564

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Abstract

An easily automatable flow-injection (FI) method for the determination of total catechins is reported. The method is based on the reaction of vanillin in acid medium to yield a coloured product with maximum absorption at 500 nm. After optimisation by the univariate and multivariate approaches as required, the linear range was established (between 10 and 90 mgL-1 and 10 and 250 mgL-1 for white and red wines, respectively). Then, the assessment of the proposed versus the reference method was studied in terms of repeatability (2.57 mgL-1), reproducibility (3.56 mgL-1) (no significant differences were found), detection and quantification limits (not far from those of the reference method and always sufficient for the determination of catechins in any type of wine), traceability (excellent correlation under all conditions) and sample throughput (23 samples h-1 for the proposed method versus 3 samples h-1 for the reference method).

Item Type:	Article
Additional Information:	An easily automatable flow-injection (FI) method for the determination of total catechins is reported. The method is based on the reaction of vanillin in acid medium to yield a coloured product with maximum absorption at 500 nm. After optimisation by the univariate and multivariate approaches as required, the linear range was established (between 10 and 90 mgL-1 and 10 and 250 mgL-1 for white and red wines, respectively). Then, the assessment of the proposed versus the reference method was studied in terms of repeatability (2.57 mgL-1), reproducibility (3.56 mgL-1) (no significant differences were found), detection and quantification limits (not far from those of the reference method and always sufficient for the determination of catechins in any type of wine), traceability (excellent correlation under all conditions) and sample throughput (23 samples h-1 for the proposed method versus 3 samples h-1 for the reference method).
Keywords:	procyanidins, catechins, flow injection, analytical chemistry, method validation, food chemistry, method optimisation, automation, spectrophotometry, wine
Subjects:	F Physical Sciences > F110 Applied Chemistry F Physical Sciences > F190 Chemistry not elsewhere classified F Physical Sciences > F100 Chemistry F Physical Sciences > F180 Analytical Chemistry
Divisions:	College of Sciences > Faculty of Science > School of Life Sciences
Depositing User:	Jose Gonzalez-Rodriguez
Date Deposited:	08 Jan 2010 09:50
Last Modified:	13 Mar 2013 08:34
URI:	http://eprints.lincoln.ac.uk/id/eprint/2118

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