Cloning and characterisation of the chicken orthologue of dendritic cell-lysosomal associated membrane protein (DC-LAMP)

Wu, Zhiguang and Hu, Tuanjun and Butter, Colin (2010) Cloning and characterisation of the chicken orthologue of dendritic cell-lysosomal associated membrane protein (DC-LAMP). Developmental and Comparative Immunology, 34 (2). pp. 183-188. ISSN 0145-305X

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Item Type:Article
Item Status:Live Archive

Abstract

A cDNA encoding the chicken orthologue of dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208 was cloned by RT-PCR from RNA isolated from mature chicken bone marrow-derived dendritic cells (chBM-DCs). The cloned chicken DC-LAMP (chDC-LAMP) cDNA consists of 1281 nucleotides encoding an open reading frame of 426 amino acids (aa). Comparison of the deduced aa sequence of DC-LAMP with orthologous proteins from human and mouse revealed 27 and 24 identity, respectively. The predicted chDC-LAMP protein shares the characteristic features of LAMP family members. ChDC-LAMP mRNA, unlike its mammalian orthologues, was expressed in a wide range of tissues, at highest levels in the lung. Lymphoid tissues including thymus, spleen, bursa, ceacal tonsil and Meckel's diverticulum had high chDC-LAMP mRNA expression levels. ChDC-LAMP mRNA was expressed in all splenocyte subsets with the highest expression in Bu-1 + B cells and KUL01 + cells, which would include macrophages and DC. ChDC-LAMP mRNA was highly expressed in chBM-DC, whereas expression levels in chicken monocyte-derived macrophages (chMo-Mac) and the HD11 macrophage cell line were significantly lower. Following CD40L stimulation, chDC-LAMP mRNA expression levels were up-regulated in mature chBM-DC, chMo-Mac and HD11 cells whereas lipopolysaccharide (LPS) only up-regulated chDC-LAMP mRNA expression levels in chBM-DC. ChDC-LAMP is not solely expressed on chicken DC but can be used as a marker to differentiate between immature and mature DC. © 2009 Elsevier Ltd. All rights reserved.

Keywords:CD40 ligand, complementary DNA, dendritic cell lysosome associated membrane protein, Escherichia coli lipopolysaccharide, messenger RNA, amino acid sequence, animal cell, animal tissue, article, chicken, controlled study, dendritic cell, Escherichia coli, gene expression regulation, lymphoid tissue, macrophage, molecular cloning, nonhuman, nucleotide sequence, open reading frame, orthology, priority journal, spleen cell, upregulation, Amino Acid Sequence, Animals, Chickens, Cloning, Molecular, Conserved Sequence, Dendritic Cells, Humans, Lysosome-Associated Membrane Glycoproteins, Macrophages, Molecular Sequence Data, Phylogeny, Sequence Alignment, Mammalia
Subjects:D Veterinary Sciences, Agriculture and related subjects > D300 Animal Science
Divisions:College of Science > School of Life Sciences
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ID Code:19857
Deposited On:01 Jan 2016 19:28

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