Live imaging of cellular internalization of single colloidal particle by combined label-free and fluorescence total internal reflection microscopy

Byrne, Gerard and Vllasaliu, Driton and Falcone, Franco H. and Somekh, Michael G. and Stolnik, Snjezana (2015) Live imaging of cellular internalization of single colloidal particle by combined label-free and fluorescence total internal reflection microscopy. Molecular Pharmaceutics, 12 (11). p. 3862. ISSN 1543-8384

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Abstract

In this work we utilise the combination of label-free total internal reflection microscopy and total internal reflectance fluorescence (TIRM/TIRF) microscopy to achieve a simultaneous, live imaging of single, label-free colloidal particle endocytosis by individual cells. The TIRM arm of the microscope enables label free imaging of the colloid and cell membrane features, while the TIRF arm images the dynamics of fluorescent-labelled clathrin (protein involved in endocytosis via clathrin pathway), expressed in transfected 3T3 fibroblasts cells. Using a model polymeric colloid and cells with a fluorescently-tagged clathrin endocytosis pathway, we demonstrate that wide field TIRM/TIRF co-imaging enables live visualization of the process of colloidal particle interaction with the labelled cell structure, which is valuable for discerning the membrane events and route of colloid internalization by the cell. We further show that 500 nm model polystyrene colloid associates with clathrin, prior to and during its cellular internalisation. This association is not apparent with larger, 1 μm colloid, indicating an upper particle size limit for clathrin-mediated endocytosis.

Keywords:Cell imaging; total internal reflection microscopy; total internal reflectance fluorescence microscopy, NotOAChecked
Subjects:B Subjects allied to Medicine > B230 Pharmacy
Divisions:College of Science > School of Pharmacy
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ID Code:18865
Deposited On:29 Sep 2015 16:34

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