Ester biosynthesis in kiwifruit: from genes to enzymes to pathways

Souleyre, Edwige J. F. and Günther, Catrin and Wang, Mindy Y. and Newcomb, Richard D. and March, Ken B. (2011) Ester biosynthesis in kiwifruit: from genes to enzymes to pathways. In: Acta Horticulturae. International Society for Horticultural Science, pp. 205-211. ISBN 978-906605604-6

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18410 ISHS Acta Horticulturae 913 abstract.pdf - Abstract

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Abstract

The distinctive flavours of different kiwifruit (Actinidia) genotypes are determined by a unique combination of volatile compounds. We are using a genomics approach to identify genes responsible for the production of esters in kiwifruit. From an extensive database of kiwifruit ESTs we have mined acyl transferase genes, including putative alcohol acyl transferases predicted to be involved in the final step of ester biosynthesis. In this paper we report the first functional characterisation of two acyl transferases in kiwifruit, AeAT9 and AdAT17, using either transient expression in planta or by recombinant protein expression in yeast. Evidence is provided that both enzymes are alcohol acyl transferases and could potentially have roles in the biosynthesis of esters in kiwifruit. Our results suggest these two alcohol acyl transferases enzymes contribute to the production of branched, straight chain and sulphur esters.

Additional Information:VII International Symposium on Kiwifruit
Keywords:Actinidia; Acyl transferase; Alcohol acyl transferase; Aroma volatiles; Ester biosynthesis, bmjtype
Subjects:C Biological Sciences > C990 Biological Sciences not elsewhere classified
Divisions:College of Social Science
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ID Code:18410
Deposited On:17 Aug 2015 16:08

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