Potentiators of insulin secretion modulate Ca2+ sensitivity in rat pancreatic islets

Hughes, S. J. and Christie, M. R. and Ashcroft, S. J. H. (1987) Potentiators of insulin secretion modulate Ca2+ sensitivity in rat pancreatic islets. Molecular and Cellular Endocrinology, 50 (3). pp. 231-236. ISSN 0303-7207

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Item Type:Article
Item Status:Live Archive

Abstract

Insulin secretion stimulated by 10 mM glucose was potentiated by forskolin, an activator of adenyl cyclase, by acetyl choline which may enhance turnover of inositol phospholipids, and by tetradecanoyl phorbol acetate (TPA), an activator of protein kinase C. None of these agents initiated insulin secretion in the presence of 2 mM glucose. Glucose-stimulated insulin secretion was markedly dependent on the concentration of extracellular Ca2+: at or below 10 μM Ca2+ no insulin secretion was evoked by glucose in freshly isolated islets. The threshold Ca2+ requirement was increased after culture of islets for 44 h. In both fresh and cultured islets the presence of a potentiator of secretion produced both a marked increase in the maximum rate of glucose-stimulated insulin secretion and a lowering of the requirement for extracellular Ca2+. Thus potentiation of insulin release involves an increase in the sensitivity of the B cell to Ca2+. © 1987.

Keywords:acetylcholine, calcium, forskolin, glucose, phorbol 13 acetate 12 myristate, phorbol ester, animal experiment, dose response, drug comparison, drug dose, drug efficacy, endocrine system, insulin release, nonhuman, pancreas, pancreas islet, pharmacokinetics, preliminary communication, priority journal, rat, Acetylcholine, Animal, In Vitro, Insulin, Islets of Langerhans, Male, Rats, Rats, Inbred Strains, Support, Non-U.S. Gov't, Tetradecanoylphorbol Acetate
Subjects:B Subjects allied to Medicine > B120 Physiology
Divisions:College of Science > School of Life Sciences
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ID Code:18173
Deposited On:08 Aug 2015 16:51

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