Autoantibodies to a 38-kDa glycosylated islet cell membrane-associated antigen in (pre)type 1 diabetes: Association with IA-2 and islet cell autoantibodies

Winnock, Frederic and Christie, Michael R. and Batstra, Manou R. and Aanstoot, Henk-Jan and Weets, Ilse and Decochez, Katelijn and Jopart, Philippe and Nicolaij, Dany and Gorus, Frans K. (2001) Autoantibodies to a 38-kDa glycosylated islet cell membrane-associated antigen in (pre)type 1 diabetes: Association with IA-2 and islet cell autoantibodies. Diabetes Care, 24 (7). pp. 1181-1186. ISSN 0149-5992

Full content URL: http://care.diabetesjournals.org/content/24/7/1181...

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Autoantibodies to a 38-kDa glycosylated islet cell membrane-associated antigen in (pre)type 1 diabetes: Association with IA-2 and islet cell autoantibodies

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Abstract

OBJECTIVE - To study the association of autoantibodies against a 38-kDa glycated islet cell membrane-associated (GLIMA) protein with (pre)type 1 diabetes, patient characteristics, and other immune and genetic markers of the disease and to evaluate the possible added value of GLIMA antibody determinations for disease prediction and classification. RESEARCH DESIGN AND METHODS - Recent-onset type 1 diabetic patients (n = 100), prediabetic siblings (n = 23), and nondiabetic control subjects (n = 100) were consecutively recruited by the Belgian Diabetes Registry. GLIMA antibodies were determined by immunoprecipitation of radiolabeled islet cell proteins; islet cell antibodies (ICAs) were determined by indirect immunofluorescence; and insulin autoantibodies (IAAs), insulinoma-associated protein-2 antibodies (IA-2As), and GAD antibodies (GADAs) were determined by radioligand assays. RESULTS - GLIMA antibodies were detected in 38% of type 1 diabetic patients and 35 of prediabetic siblings (during follow-up) vs. 0% in control subjects (P < 0.001). Their prevalence was lower than that of other antibodies and was significantly associated with high levels of 1A-2A and ICA (P < 0.0001). In (pre)diabetes, GLIMA antibodies could only be demonstrated in sera positive for ≥1 other autoantibody. CONCLUSIONS - GLIMA antibodies are strongly associated with type 1 diabetes and antibody markers of rapid progression to clinical onset but have a lower diagnostic sensitivity for the disease than IAA, ICA, IA-2A, or GADA. In its present form, the GLIMA antibody assay does not provide much additional information for prediction or classification of diabetes, compared with that obtained from the measurement of IA-2As alone or in combination with IAAs, ICAs, and GADAs.

Keywords:autoantibody, glutamate decarboxylase, HLA DQ antigen, ICA512 autoantibody, pancreas islet cell antibody, adolescent, adult, article, Belgium, blood, child, comparative study, female, genetic polymorphism, genetics, genotype, glycosylation, human, immunology, impaired glucose tolerance, infant, insulin dependent diabetes mellitus, male, nuclear family, pancreas islet, prediction and forecasting, preschool child, register, sexual development, Adolescent, Autoantibodies, Child, Preschool, Diabetes Mellitus, Type 1, HLA-DQ Antigens, Humans, Islets of Langerhans, Polymorphism, Genetic, Prediabetic State, Predictive Value of Tests, Registries, Sex Characteristics
Subjects:A Medicine and Dentistry > A100 Pre-clinical Medicine
Divisions:College of Science > School of Life Sciences
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ID Code:18136
Deposited On:07 Aug 2015 09:54

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