Evidence for the presence of stem cell-like progenitor cells in human adult pancreas

Zhao, Min and Amiel, Stephanie A. and Christie, Michael R. and Muiesan, Paolo and Srinivasan, Parthi and Littlejohn, Wendy and Rela, Mohamed and Arno, Matthew and Heaton, Nigel and Huang, Guo Cai (2007) Evidence for the presence of stem cell-like progenitor cells in human adult pancreas. Journal of Endocrinology, 195 (3). pp. 407-414. ISSN 0022-0795

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Evidence for the presence of stem cell-like progenitor cells in human adult pancreas

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Abstract

The origin of cells replacing ageing β-cells in adult life is unknown. This study assessed the expression of classic stem cell markers: Oct4, Sox2 and CD34 in islet-enriched fractions versus exocrine cell-enriched fractions from 25 adult human pancreases following human islet isolation. Expression of Oct4, Sox2 and CD34 mRNAs was found in all cell samples, with no significant differences between endocrine and exocrine cell fractions. Immunohistochemical staining for Oct4, Sox2, CD133, CD34, CK19, insulin and nestin on human pancreas sections showed that the majority of Oct4+ve cells were found in the walls of small ducts. Similar localisations were observed for Sox2+ve cells. The majority of Sox2+ve cells were found to co-express Oct4 proteins, but not vice versa. Cells positive for Oct4 and Sox2 appeared to be a unique cell population in the adult human pancreases without co-expression for CK19, CD34, CD133, insulin and nestin proteins. The numbers of Oct4+ve and Sox2+ve cells varied among donors and were ∼1–200 and 1–30 per 100 000 pancreatic cells respectively.

Keywords:CD133 antigen, CD34 antigen, cytokeratin 19, insulin, messenger RNA, nestin, octamer transcription factor 4, transcription factor Sox2, biological marker, glycoprotein, high mobility group B protein, leukocyte antigen, peptide, POU5F1 protein, human, SOX2 protein, human, transcription factor, unclassified drug, adult, article, cell aging, cell count, cell isolation, cell population, cell specificity, cell type, cellular distribution, clinical article, controlled study, exocrine cell, female, gene expression, human, human cell, human tissue, immunohistochemistry, male, organ donor, pancreas duct, pancreas islet beta cell, pancreas islet cell, priority journal, protein expression, stem cell, aging, chemistry, cytology, donor, genetics, metabolism, methodology, middle aged, pancreas, reverse transcription polymerase chain reaction, staining, Adult, Antigens, CD, Antigens, CD34, Biological Markers, Glycoproteins, HMGB Proteins, Humans, Octamer Transcription Factor-3, Pancreatic Ducts, Peptides, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger, Staining and Labeling, Stem Cells, Tissue Donors, Transcription Factors
Subjects:B Subjects allied to Medicine > B120 Physiology
Divisions:College of Science > School of Life Sciences
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ID Code:18119
Deposited On:05 Aug 2015 14:22

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