Islets in early life are resistant to detrimental effects of a high-fat maternal diet: a study in rats

Foot, V. L. and Richardson, C. C. and Jefferson, W. and Taylor, P. D. and Christie, Michael R. (2010) Islets in early life are resistant to detrimental effects of a high-fat maternal diet: a study in rats. Hormone and Metabolic Research, 42 (13). pp. 923-929. ISSN 0018-5043

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Item Type:Article
Item Status:Live Archive

Abstract

Offspring of rats fed high-fat diets during pregnancy and lactation develop glucose intolerance and islet dysfunction in adulthood. Because other models of developmental programming of glucose intolerance are associated with defective islet development, we investigated whether high-fat exposure during fetal or neonatal life impairs islet development and function, thereby contributing to islet dysfunction in later life. Female rats were fed control or high-fat diets and their pups cross-fostered after birth to represent 4 groups with each combination of control and high-fat diet for the natural and foster mother. In a time course study, pups were kept with the natural mother until weaning. Pancreases were analysed for insulin content, beta cell mass, and islet number. Isolated islets were studied for insulin secretory responses and susceptibility to palmitate-induced apoptosis assessed by caspases 3/9 activity. Pancreatic insulin content and beta cell mass were increased in pups exposed to maternal high-fat diets after birth, whereas glucose-stimulated insulin secretion from islets of high-fat offspring at 5 and 11 days of age was lower than controls. Islets from control rats of 2-14 days of age were resistant to the pro-apoptotic effects of palmitate seen in older animals. The immature beta cell is therefore insensitive to toxic effects of palmitate and may compensate for the inhibitory effects on insulin secretion by increasing beta cell mass. The data suggest that susceptibility to glucose intolerance in offspring of dams fed high-fat diets may not be a consequence of deleterious effects on beta cell mass in early life. © Georg Thieme Verlag KG Stuttgart - New York.

Keywords:caspase, glucose, insulin, palmitic acid, animal experiment, apoptosis, article, cell count, cell function, cell maturation, controlled study, female, fetus, fetus development, glucose intolerance, insulin release, lipid diet, maternal nutrition, newborn, newborn period, nonhuman, pancreas, pancreas islet beta cell, priority journal, progeny, rat, suckling, weaning, Animals, Animals, Newborn, Animals, Suckling, Caspase 3, Caspase 9, Cytokines, Diet, Dietary Fats, Fatty Acids, Islets of Langerhans, Organ Size, Pregnancy, Prenatal Exposure Delayed Effects, Rats, Rats, Sprague-Dawley, Time Factors, Animalia, Rattus
Subjects:B Subjects allied to Medicine > B120 Physiology
Divisions:College of Science > School of Life Sciences
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ID Code:18113
Deposited On:05 Aug 2015 13:40

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