Optical detection of photo-activated protein adsorption using Brewster angle microscopy

Li, Y. and Song, H. Y. and Lear, M. J. and Gorelik, S. and Tomczak, N. and Hobley, J. (2008) Optical detection of photo-activated protein adsorption using Brewster angle microscopy. In: PhotonicsGlobal@Singapore, 2008. IPGC 2008, 8-11 Dec 2008, Singapore.

Full content URL: http://dx.doi.org/10.1109/IPGC.2008.4781381

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Abstract

Bovine serum albumin (BSA) was space selectively immobilized onto a quartz substrate using photo-reactions initiated by pulsed laser irradiation of benzophenone derivatives tethered by silane coupling to the quartz. The excitation of the benzophenone with 266 nm light yields the pi-pi* triplet state with a quantum yield of 1. This triplet state then reacts with BSA in buffer solution in contact with the modified quartz. The triplet state may form a ketyl radical by reaction with the solution or it may abstract a proton directly from the BSA becoming covalently tethered to the surface. The entire deposition process was monitored in real time, in-situ using a Brewster angle microscope (BAM).

Keywords:Immobilisation chemistry, Brewster angle microscopy
Subjects:F Physical Sciences > F170 Physical Chemistry
F Physical Sciences > F160 Organic Chemistry
Divisions:College of Science > School of Chemistry
ID Code:17199
Deposited On:23 Apr 2015 13:40

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