Pyrosequencing reveals regional differences in fruit-associated fungal communities

Taylor, Michael W. and Tsai, Peter and Anfang, Nicole and Ross, Howard A. and Goddard, Matthew R. (2014) Pyrosequencing reveals regional differences in fruit-associated fungal communities. Environmental Microbiology, 16 (9). pp. 2848-2858. ISSN 1462-2912

Full content URL: http://onlinelibrary.wiley.com/doi/10.1111/1462-29...

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Pyrosequencing reveals regional differences in fruit-associated fungal communities
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Abstract

We know relatively little of the distribution of microbial communities generally. Significant work has examined a range of bacterial communities, but the distribution of microbial eukaryotes is less well characterized. Humans have an ancient association with grape vines (Vitis vinifera) and have been making wine since the dawn of civilization, and fungi drive this natural process. While the molecular biology of certain fungi naturally associated with vines and wines is well characterized, complementary investigations into the ecology of fungi associated with fruiting plants is largely lacking. DNA sequencing technologies allow the direct estimation of microbial diversity from a given sample, avoiding culture-based biases. Here, we use deep community pyrosequencing approaches, targeted at the 26S rRNA gene, to examine the richness and composition of fungal communities associated with grapevines and test for geographical community structure among four major regions in New Zealand (NZ). We find over 200 taxa using this approach, which is 10-fold more than previously recovered using culture-based methods. Our analyses allow us to reject the null hypothesis of homogeneity in fungal species richness and community composition across NZ and reveal significant differences between major areas. © 2014 The Authors.

Additional Information:This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Keywords:Bacteria (microorganisms), Eukaryota, Fungi, Vitaceae, Vitis vinifera
Subjects:D Veterinary Sciences, Agriculture and related subjects > D711 Agricultural Microbiology
C Biological Sciences > C180 Ecology
Divisions:College of Science > School of Life Sciences
ID Code:17146
Deposited On:15 May 2015 09:34

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