Rapid and sensitive profiling of tear wax ester species using high performance liquid chromatography coupled with tandem mass spectrometry

Lam, Sin Man and Tong, Louis and Reux, Bastien and Lear, Martin J. and Wenk, Markus R. and Shui, Guanghou (2013) Rapid and sensitive profiling of tear wax ester species using high performance liquid chromatography coupled with tandem mass spectrometry. Journal of Chromatography A, 1308 . pp. 166-171. ISSN 0021-9673

Full content URL: http://dx.doi.org/10.1016/j.chroma.2013.08.016

Documents
JC-A-2013.pdf

Request a copy
[img] PDF
JC-A-2013.pdf - Whole Document
Restricted to Repository staff only

1MB
Item Type:Article
Item Status:Live Archive

Abstract

A rapid and sensitive method was developed for quantitative profiling of wax esters (WEs) in human tear lipide. Individual WE species was separated by liquid chromatography and detected by electrospray ionisation mass spectrometry using specific multiple reaction monitoring (MRM) scanning. Palmitoyl palmitate and in-house synthesized wax esters 13C18:1(oleic acid-1,2,3,7,8,9,10-13C7)C26:0 were used as internal standards for quantitation of WEs containing saturated and unsaturated fatty acids (FA), respectively. The limit of detection was approximately 70 nmol/L. The linearity range of the liquid chromatography (LC)-MRM detection for WEs was about three orders of magnitude. Quantitative analyses of 141 individual WE in the human tear lipidome demonstrated that species comprising FA18:1 and FA16:1 each accounted for 47.7% and 24.0% (molar%) of total WE, while fatty alcohols in WEs of human tears ranged from 17 carbons to 32 carbons with predominant species represented by C24, C25 and C26.

Keywords:Tear, Wax esters, Mass spectrometry, Multiple reaction monitoring (MRM)
Subjects:F Physical Sciences > F180 Analytical Chemistry
Divisions:College of Science > School of Chemistry
ID Code:17131
Deposited On:23 Apr 2015 12:46

Repository Staff Only: item control page