Identification of the binding of sceptrin to MreB via a bidirectional affinity protocol

Rodríguez, Abimael D. and Lear, Martin J. and La Clair, James J. (2008) Identification of the binding of sceptrin to MreB via a bidirectional affinity protocol. Journal of the American Chemical Society, 130 (23). pp. 7256-7258. ISSN 0002-7863

Full content URL: http://dx.doi.org/10.1021/ja7114019

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Abstract

While the structures of a myriad of natural products have been elucidated from marine sponges, the biological targets for only a small percentage of these materials are known. Arguably, understanding the target of these compounds can be as essential to clinic entry as the structure. In an effort to expand the scope of affinity data, we developed a system that identifies protein targets in parallel with natural product isolation. Our study focused on evaluating sponge extracts for natural products that bind to Escherichia coli proteins as a model.

Keywords:Binding affinity screening, Natural products, Biotarget identification, Fluorescent immuno-affinity label
Subjects:F Physical Sciences > F165 Biomolecular Chemistry
F Physical Sciences > F160 Organic Chemistry
F Physical Sciences > F163 Bio-organic Chemistry
Divisions:College of Science > School of Chemistry
ID Code:17118
Deposited On:16 Apr 2015 14:43

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