A study to detect Helicobacter pylori in fresh and archival specimens from patients with interstitial cystitis, using amplification methods

Agarwal, M. and Dixon, Ron (2003) A study to detect Helicobacter pylori in fresh and archival specimens from patients with interstitial cystitis, using amplification methods. BJU International, 91 (9). pp. 814-816. ISSN 1464-4096

Documents
A study to detect Helicobacter pylori in fresh and archival specimens from patients with interstitial cystitis, using amplification methods

Request a copy
[img] PDF
__ddat02_staffhome_jpartridge_j.1464-410X.2003.04221.x.pdf - Whole Document
Restricted to Repository staff only

69kB
Item Type:Article
Item Status:Live Archive

Abstract

OBJECTIVES: To detect Helicobacter pylori DNA in fresh and paraffin-embedded bladder biopsy specimens, and thus determine any possible role in interstitial cystitis (IC). MATERIALS AND METHODS: Thirty-three bladder biopsy samples were examined from patients with IC (29 paraffin-embedded and four freshly frozen) diagnosed according to National Institute of Diabetes, Digestive and Kidney Disease criteria. The positive control was a gastric biopsy sample from a patient with a known gastric ulcer caused by H. pylori infection. RESULTS: The anticipated polymerase chain reaction product size of 109 base pairs was obtained with the positive control, whereas none of the other biopsy samples (paraffin embedded or fresh) showed positive amplification specific for H. pylori. CONCLUSIONS: As there was no H. pylori DNA in any of the samples from patients with IC, it is an unlikely candidate in the pathogenesis of IC.

Keywords:bacterial DNA, paraffin, article, bacterial genetics, base pairing, bladder biopsy, clinical article, controlled study, embedding, frozen section, gene amplification, Helicobacter infection, Helicobacter pylori, histology, histopathology, human, human tissue, interstitial cystitis, polymerase chain reaction, priority journal, stomach biopsy, stomach ulcer, tissue section, Biopsy, Cystitis, Interstitial, DNA, Bacterial, Helicobacter Infections, Humans, Nucleic Acid Amplification Techniques
Subjects:C Biological Sciences > C521 Medical Microbiology
Divisions:College of Science > School of Life Sciences
ID Code:17080
Deposited On:15 Apr 2015 13:14

Repository Staff Only: item control page