Permeabilising effects of sub-inhibitory concentrations of microcystin on the growth of Escherichia coli

Dixon, Ron and Al-Nazawi, M. and Alderson, G. (2004) Permeabilising effects of sub-inhibitory concentrations of microcystin on the growth of Escherichia coli. FEMS Microbiology Letters, 230 (2). pp. 167-170. ISSN 0378-1097

Full content URL: http://femsle.oxfordjournals.org/content/230/2/167

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Permeabilising effects of sub-inhibitory concentrations of microcystin on the growth of Escherichia coli

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Abstract

Microcystin, a cyanotoxin produced by Microcystis aeruginosa, lacks potent antibacterial activity. When tested in combination, in vitro, inhibitory values for a range of hydrophobic antibiotics were significantly reduced in the presence of at least 1/20x the minimum inhibitory concentration of microcystin. The degree of inhibition was equivalent to that of a well-characterised permeabilising agent, polymyxin B nonapeptide. The permeabilising ability of sub-inhibitory concentrations of microcystin to affect the envelope of Escherichia coli was demonstrated by a rapid and sustained reduction in absorbance values of lysozyme-treated cells and by enhanced uptake of crystal violet in microcystin-treated cultures. Direct effects of appropriate concentrations of microcystin on the integrity of bacterial outer and inner membranes were measured by release of specific enzyme markers. Although the exact mechanism for permeabilising E. coli with microcystin has not been elucidated, the effects were consistent with permeability changes to the enterobacterial outer membrane caused by polymyxin B nonapeptide. © 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Keywords:crystal violet, cyanoginosin, lysozyme, polymyxin B nonapeptide, antiinfective agent, cyclopeptide, drug derivative, polymyxin B, absorption, antibacterial activity, article, bacterial growth, bacterial membrane, bacterial outer membrane, cell permeabilization, concentration response, drug effect, Enterobacteriaceae, Escherichia coli, Microcystis aeruginosa, minimum inhibitory concentration, nonhuman, priority journal, cell membrane permeability, dose response, drug potentiation, growth, development and aging, hydrophobicity, microbiological examination, Bacteria (microorganisms), Microcystis, Viola (angiosperm), Anti-Bacterial Agents, Anti-Infective Agents, Dose-Response Relationship, Drug, Drug Synergism, Microbial Sensitivity Tests, Microcystins, Peptides, Cyclic, Polymyxin B
Subjects:C Biological Sciences > C500 Microbiology
Divisions:College of Science > School of Life Sciences
ID Code:17078
Deposited On:15 Apr 2015 13:42

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