Species-specific physiological response of dinoflagellates to quantified small-scale turbulence

Berdalet, Elisa and Peters, Francesc and Koumandou, V. Lila and Roldan, Cristina and Guadayol, Oscar and Estrada, Marta (2007) Species-specific physiological response of dinoflagellates to quantified small-scale turbulence. Journal of Phycology, 43 (5). pp. 965-977. ISSN 0022-3646

Full content URL: http://dx.doi.org/10.1111/j.1529-8817.2007.00392.x

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Species-specific physiological response of dinoflagellates to quantified small-scale turbulence

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Abstract

Turbulence has been shown to alter different aspects of the physiology of some dinoflagellates. The response appears to be species-specific and dependent on the experimental design and setup used to generate small-scale turbulence. We examined the variability of the response of three dinoflagellate species to the turbulence, following the same experimental design used by Berdalet (1992) on Akashiwo sanguinea (Hirasaka) Ge. Hansen et Moestrup (=Gymnodinium nelsonii G. W. Martin). In all experiments, turbulence was generated by an orbital shaker at 100 rpm, which corresponded on bulk average, to dissipation rates (epsilon, quantified using an acoustic Doppler velocimeter) of approximate to 2 cm(2) . s(-3). Turbulence did not appreciably affect Gymnodinium sp., a small dinoflagellate. However, Alexandrium minutum Halim and Prorocentrum triestinum J. Schiller exhibited a reduced net growth rate (33% and 28%, respectively) when shaken during the exponential growth phase. Compared to the still cultures, the shaken treatments of A. minutum and P. triestinum increased the mean cell volume (up to 1.4- and 2.5-fold, respectively) and the mean DNA content (up to 1.8- and 5.3-fold, respectively). Cultures affected by turbulence recovered their normal cell properties when returned to still conditions. The swimming speed of the cells exposed to agitation was half that of the unshaken ones. Overall, the response of A. minutum and P. triestinum was similar, but with lower intensity, to that observed previously on A. sanguinea. We found no clear trends related to taxonomy or morphology.

Keywords:cellular DNA conten, dinoflagellates, net growth rate, orbital shaking, small-scale turbulence, swimming speed}
Subjects:C Biological Sciences > C180 Ecology
C Biological Sciences > C161 Marine Biology
H Engineering > H141 Fluid Mechanics
F Physical Sciences > F700 Ocean Sciences
C Biological Sciences > C500 Microbiology
Divisions:College of Science > School of Life Sciences
ID Code:16477
Deposited On:13 Feb 2015 11:09

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