IL-1beta-dependent activation of NF-kappaB mediates PGE2 release via the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase

Catley, Matthew C. and Chivers, Joanna E. and Cambridge, Lisa M. and Holden, Neil and Slater, Donna M. and Staples, Karl J. and Bergmann, Martin W. and Loser, Peter and Barnes, Peter J. and Newton, Robert (2003) IL-1beta-dependent activation of NF-kappaB mediates PGE2 release via the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase. FEBS Letters, 547 (1-3). pp. 75-79. ISSN 0014-5793

Full content URL: http://dx.doi.org/10.1016/S0014-5793(03)00672-0

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Item Type:Article
Item Status:Live Archive

Abstract

Prostaglandin (PG) E2 release is induced in pulmonary A549 cells by the NF-kappaB-activating stimuli interleukin-1beta (IL-1beta) and phorbol 12-myristate 13-acetate (PMA). Adenoviral over-expression of IkappaBalphaDeltaN, a dominant NF-kappaB inhibitor, prevents NF-kappaB-dependent transcription and was used to qualify the role of NF-kappaB in the release of PGE2. IkappaBalphaDeltaN repressed IL-1beta-induced, but not PMA-induced, cycloxygenase-2 (COX-2) and microsomal prostaglandin E synthase (mPGES) expression. These data conclusively demonstrate a substantial role for NF-kappaB in the co-ordinate induction of COX-2, mPGES and in the corresponding release of PGE2 by IL-1beta. However, other pathways are primarily responsible for PGE2 release induced by PMA.

Keywords:Prostaglandins, Prostaglandin E synthetase, Nuclear factor ?B, Prostaglandin GH synthase, Cyclooxygenase-2
Divisions:College of Science > School of Life Sciences
ID Code:15133
Deposited On:09 Jun 2015 08:30

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