ATP-induced intracellular Ca2+ signals in isolated human insulin-secreting cells

Squires, Paul E. and James, R. F. L. and London, N. J. M. and Dunne, M. J. (1994) ATP-induced intracellular Ca2+ signals in isolated human insulin-secreting cells. Pflugers Archiv European Journal of Physiology, 427 (1-2). pp. 181-183. ISSN 0031-6768

Full content URL: http://link.springer.com/article/10.1007%2FBF00585...

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Item Type:Article
Item Status:Live Archive

Abstract

Using isolated β-cells from human islets of Langerhans we have demonstrated that purinergic receptor agonists are functionally coupled to rises in the intracellular calcium ion concentration (Ca2+i). The effects of ATP, ADP and AMP have been examined over a range of concentrations, 0.5 to 500μM. The actions of ATP were more potent than those of either ADP or AMP suggesting that a P2-type of purinergic receptor operates in these cells. Responses to ATP were concentration-related, but exhibited marked desensitisation at high concentrations (>100μM). Purinergic receptor agonists elevate Ca2+i by mechanisms that involve both Ca2+ influx and Ca2+ mobilisation from intracellular stores. The physiological significance of our data has been discussed, and related to previous studies carried out upon rodent and clonal insulin-secreting cells. © 1994 Springer-Verlag.

Keywords:2,5 di tert butylhydroquinone, adenosine diphosphate, adenosine phosphate, adenosine triphosphate, caffeine, potassium chloride, article, calcium cell level, controlled study, human, human cell, pancreas islet beta cell, priority journal, Adenosine Diphosphate, Adenosine Monophosphate, Calcium, Cell Separation, Fura-2, Insulin, Intracellular Membranes, Islets of Langerhans, Signal Transduction, Support, Non-U.S. Gov't
Subjects:C Biological Sciences > C130 Cell Biology
Divisions:College of Science > School of Life Sciences
ID Code:14514
Deposited On:16 Jul 2014 15:24

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