Effect of laser treatment on the attachment and viability of mesenchymal stem cell responses on shape memory NiTi alloy

Chan, C. W. and Hussain, Issam and Waugh, David and Lawrence, Jonathan and Man, H. C. (2014) Effect of laser treatment on the attachment and viability of mesenchymal stem cell responses on shape memory NiTi alloy. Materials Science and Engineering C, 42 . pp. 254-263. ISSN 0928-4931

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Abstract

The objectives of this study were to investigate the effect of laser-induced surface features on the morphology, attachment and viability of mesenchymal stem cells (MSCs) at different periods of time, and to evaluate the biocompatibility of different zones: laser-melted zone (MZ), heat-affected zone (HAZ) and base metal (BM) in laser-treated NiTi alloy. The surface morphology and composition were studied by scanning electron microscope (SEM) and X-ray photoemission spectroscopy (XPS), respectively. The cell morphology was examined by SEM while the cell counting and viability measurements were done by hemocytometer and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. The results indicated that the laser-induced surface features, such as surface roughening, presence of anisotropic dendritic pattern and complete surface Ni oxidation were beneficial to improve the biocompatibility of NiTi as evidenced by the highest cell attachment (4 days of culture) and viability (7 days of culture) found in the MZ. The biocompatibility of the MZ was the best, followed by the BM with the HAZ being the worst. The defective and porous oxide layer as well as the coarse grained structure might attribute to the inferior cell attachment (4 days of culture) and viability (7 days of culture) on the HAZ compared with the BM which has similar surface morphology. © 2014 Elsevier B.V.

Keywords:Biocompatibility, Cells, Fiber lasers, Heat affected zone, Morphology, Photoelectron spectroscopy, Scanning electron microscopy, Surface morphology, Coarse-grained structure, Laser treatment, Mesenchymal stem cell, Morphology and composition, NiTi, Shape memory alloys(SMA), Shape memory Ni-Ti alloys, X ray photoemission spectroscopy, Cell culture, NotOAChecked
Subjects:F Physical Sciences > F200 Materials Science
Divisions:College of Science > School of Life Sciences
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ID Code:14388
Deposited On:11 Jul 2014 08:18

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