L-type calcium channels regulate gastrin release from human antral G cells

Ray, J. M. and Squires, Paul and Meloche, R. M. and Nelson, D. W. and Snutch, T. P. and Buchan, A. M. J. (1997) L-type calcium channels regulate gastrin release from human antral G cells. American Journal of Physiology - Gastrointestinal and Liver Physiology, 273 (2). G281-G288. ISSN 0193-1857

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Item Type:Article
Item Status:Live Archive

Abstract

In mRNA samples isolated from a gastrin (G) cell-enriched human antral cell preparation, reverse transcription-polymerase chain reaction identified products encoding part of the alpha 1-subunit of class C and D L-type voltage- dependent Ca 2+ channels (VDCCs). Analysis of the polymerase chain reaction products demonstrated a 100 homology with the known human gene sequences. An antibody to the class D alpha 1-subunit immunostained 30-40 of the cultured cells; of these 90 were gastrin immunoreactive. Gastrin release stimulated by terbutaline (beta 2-agonist) and forskolin was abolished by blockade of L- type VDCCs; the effect of 3.6 mM extracellular Ca 2+ was only partially reversed. In G cells the rise in intracellular Ca 2+ observed in response to increasing extracellular Ca 2+ from 0.5 to 3.6 mM was reduced by nitrendipine. These results indicated that human antral cells expressed class C and D L-type VDCCs. Activation of G cells with beta-adrenergic agonists required an influx of extracellular Ca 2+ through these channels to stimulate gastrin release. However, activation of L-type channels was not the only mechanism underlying Ca 2+-stimulated gastrin release.

Keywords:calcium channel, calcium ion, gastrin, article, calcium cell level, extracellular calcium, gastrin release, gene sequence, hormone determination, human, human cell, priority journal, reverse transcription polymerase chain reaction, sequence homology, stomach antrum, Adrenergic beta-Agonists, Adult, Base Sequence, Calcium, Calcium Channel Blockers, Calcium Channels, Female, Fluorometry, Forskolin, Gastrins, Humans, Immunohistochemistry, Male, Molecular Sequence Data, Nitrendipine, Polymerase Chain Reaction, Pyloric Antrum, Terbutaline, Transcription, Genetic
Subjects:C Biological Sciences > C130 Cell Biology
Divisions:College of Science > School of Life Sciences
ID Code:14357
Deposited On:18 Jul 2014 13:02

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