Pancreatic β-cell-to-β-cell interactions are required for integrated responses to nutrient stimuli: enhanced Ca 2+ and insulin secretory responses of MIN6 pseudoislets

Hauge-Evans, A. C. and Squires, Paul E. and Persaud, S. J. and Jones, P. M. (1999) Pancreatic β-cell-to-β-cell interactions are required for integrated responses to nutrient stimuli: enhanced Ca 2+ and insulin secretory responses of MIN6 pseudoislets. Diabetes, 48 (7). pp. 1402-1408. ISSN 0012-1797

Full content URL: http://diabetes.diabetesjournals.org/content/48/7/...

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Item Type:Article
Item Status:Live Archive

Abstract

The effect of cell-to-cell contact on Ca 2+ influx and secretory responses in the β-cell line MIN6 was studied using MIN6 pseudoislets, which are three-dimensional islet-like cell aggregates that develop when MIN6 cells are cultured for 6-8 days on gelatin. The formation of pseudoislets is dependent on the Ca 2+-dependent adhesion molecule E-cadherin (E-CAD), since the process can be inhibited by incubation in the absence of Ca 2+ or in the presence of an anti-E-CAD antibody. Glucose and a-ketoisocaproic acid (KIC) evoked a Ca 2+ influx in only a small fraction of the MIN6 monolayer cells, whereas >80% of cell groups within the pseudoislets responded to both nutrients. In contrast, changes in the intracellular free Ca2+ concentration (Ca2+i) were observed in all or most monolayer cells or pseudoislet cell groups in response to physical or pharmacological depolarizing stimuli. No significant increase in insulin release was observed from MIN6 monolayer cells in response to nutrient or nonnutrient insulin secretagogues. Conversely, pseudoislets were found to respond significantly to both nutrients and nonnutrients. These results suggest that close cell-to-cell contact improves the functional responsiveness of MIN6 cells and that pseudoislets may therefore serve as a useful research model in the study of β-cell function.

Keywords:2 oxoisocaproic acid, calcium ion, glucose, insulin, uvomorulin, article, calcium transport, cell aggregation, cell interaction, controlled study, insulin release, nutrient, pancreas islet beta cell, priority journal, Calcium, Cell Communication, Cell Line, Transformed, Humans, Islets of Langerhans, Nutritional Status, Secretory Rate, Stimulation, Chemical
Subjects:C Biological Sciences > C990 Biological Sciences not elsewhere classified
Divisions:College of Science > School of Life Sciences
ID Code:14354
Deposited On:18 Jun 2014 15:21

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