Human granulosa-lutein cells express functional EP1 and EP2 prostaglandin receptors

Harris, T. E. and Squires, Paul E. and Michael, A. E. and Bernal, A. L. and Abayasekara, D. Robert (2001) Human granulosa-lutein cells express functional EP1 and EP2 prostaglandin receptors. Biochemical and Biophysical Research Communications, 285 (5). pp. 1089-1094. ISSN 0006-291X

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Item Type:Article
Item Status:Live Archive

Abstract

Prostaglandin E2 (PGE2) exerts mainly luteotrophic effects in the corpus luteum. In other tissues, PGE2 acts via specific PGE2 receptor subtypes including EP1, which modulates intracellular calcium (Ca2+i) and EP2, which is coupled to cyclic AMP (cAMP) generation. We have therefore investigated the presence of functional EP1 and EP2 receptors using human granulosa-lutein (GL) cells. Reverse-transcription PCR revealed that GL cells expressed mRNA transcripts encoding both EP1 and EP2 receptors. When GL cells were challenged with ligands that can bind to both receptor subtypes (PGE2 and 16,16 dimethyl PGE2) or exclusively to EP2 (butaprost), both cAMP formation and progesterone synthesis were stimulated. Furthermore, the cAMP response to these agonists could be significantly blocked by an EP1/2 antagonist AH6809 but not by an EP1-selective antagonist SC19220. Exposure of GL cells to 16,16-dm PGE2 transiently raised Ca2+i levels, which could be prevented by both AH6809 and SC19220. We therefore conclude that human GL cells express functional EP1 and EP2 receptors. © 2001 Academic Press.

Keywords:1 acetyl 2 (8 chloro 10,11 dihydrodibenzb,f1,4oxazepine 10 carbonyl)hydrazine, 6 isopropoxy 9 oxo 2 xanthenecarboxylic acid, cyclic AMP, progesterone, prostaglandin E2, prostaglandin ep 1 receptor, prostaglandin ep 2 receptor, prostaglandin receptor, receptor subtype, unclassified drug, article, granulosa cell, human, human cell, luteal cell, priority journal, protein expression, receptor binding, reverse transcription polymerase chain reaction, signal transduction, 16,16-Dimethylprostaglandin E2, Alprostadil, Calcium, Cells, Cultured, Dibenz(b,f)(1,4)oxazepine-10(11H)-carboxylic acid, 8-chloro-, 2-acetylhydrazide, Dinoprostone, Dose-Response Relationship, Drug, Female, Granulosa Cells, Humans, Intracellular Fluid, Lutein, Prostaglandin Antagonists, Receptors, Prostaglandin E, RNA, Messenger, Xanthenes, Xanthones
Subjects:C Biological Sciences > C990 Biological Sciences not elsewhere classified
Divisions:College of Science > School of Life Sciences
ID Code:14330
Deposited On:13 Jun 2014 11:08

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