Similarities of K+ ATP channel expression and Ca 2+ changes in pancreatic β-cells and hypothalamic neurons

Squires, Paul E. and Churamani, D. and Pararajasingam, R. and Persaud, S. J. and Jones, P. M. (2005) Similarities of K+ ATP channel expression and Ca 2+ changes in pancreatic β-cells and hypothalamic neurons. Pancreas, 30 (3). pp. 227-232. ISSN 0885-3177

Full content URL: http://dx.doi.org/10.1097/01.mpa.0000153614.55761....

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Abstract

The mechanisms through which pancreatic β cells recognize and respond to changes in circulating glucose are well understood. Evidence is accumulating that a subpopulation of neurons in the ventromedial hypothalamus (VMH) use similar cellular mechanisms to sense changes in extracellular glucose. In the present study, we used PCR and single-cell calcium imaging techniques to investigate whether glucose-sensing cells in the pancreas and hypothalamus employ a similar set of stimulus-response elements. Dispersed cells from mouse pancreata and hypothalamus were used in conjunction with the insulin-secreting cell line MIN6. We present functional data suggesting that both pancreatic and a subpopulation of hypothalamic cells exhibit glucose- and tolbutamide-evoked changes in cytosolic calcium and consider some clinical implications of different glucose sensors using the same mechanisms. Copyright © 2005 by Lippincott Williams & Wilkins.

Keywords:adenosine triphosphate, calcium ion, potassium channel, potassium ion, animal cell, animal tissue, article, calcium cell level, cell line, controlled study, hypothalamus, mouse, nonhuman, pancreas islet beta cell, polymerase chain reaction, priority journal, stimulus response, zona incerta, Animals, ATP-Binding Cassette Transporters, Calcium, Glucose, Hypoglycemic Agents, Insulin-Secreting Cells, Mice, Mice, Inbred Strains, Multidrug Resistance-Associated Proteins, Neurons, Potassium Channels, Inwardly Rectifying, Potassium Chloride, RNA, Messenger, Tolbutamide, Ventromedial Hypothalamic Nucleus
Subjects:C Biological Sciences > C900 Others in Biological Sciences
Divisions:College of Science > School of Life Sciences
ID Code:14260
Deposited On:06 Jun 2014 08:39

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