Quantitative investigation of calcimimetic R568 on beta-cell adhesion and mechanics using AFM single-cell force spectroscopy

Siamantouras, Eleftherios and Hills, Claire E. and Younis, Mustafa Y. G. and Squires, Paul and Liu, Kuo-Kang (2014) Quantitative investigation of calcimimetic R568 on beta-cell adhesion and mechanics using AFM single-cell force spectroscopy. FEBS Letters, 588 (7). pp. 1178-1183. ISSN 0014-5793

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Abstract

In this study we use a novel approach to quantitatively investigate mechanical and interfacial
properties of clonal b-cells using AFM-Single Cell Force Spectroscopy (SCFS). MIN6 cells were incubated
for 48 h with 0.5 mMCa2+ ± the calcimimetic R568 (1 lM). AFM-SCFS adhesion and indentation
experiments were performed by using modified tipless cantilevers. Hertz contact model was applied
to analyse force–displacement (F–d) curves for determining elastic or Young’s modulus (E). Our
results show CaSR-evoked increases in cell-to-cell adhesion parameters and E modulus of single
cells, demonstrating that cytomechanics have profound effects on cell adhesion characterization.

Keywords:AFM-SCFS, Cell adhesion, Indentation, Elasticity, CaSR, Calcimimetic, NotOAChecked
Subjects:C Biological Sciences > C130 Cell Biology
H Engineering > H140 Mechanics
Divisions:College of Science > School of Life Sciences
ID Code:14098
Deposited On:20 May 2014 13:45

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