Cryopreservation of mammalian semen

Curry, Mark (2007) Cryopreservation of mammalian semen. In: Cryopreservation and freeze-drying protocols. Methods in molecular biology, 368 . Humana Press, Totowa, N.J, USA, pp. 303-311. ISBN 1597453625

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Abstract

Mammalian spermatozoa were among the very first cells to be successfully cryopreserved and over the last five decades the use of frozen-thawed semen for artificial insemination has come to play an important role in domestic livestock production. More recently, semen freezing has increasingly been utilized in the establishment of genetic resource banks for endangered species. Semen is collected, most commonly either by use of an artificial vagina or by electroejaculation of an anaesthetized animal, and basic sperm parameters assessed. Semen is extended using a TEST-egg yolk-glycerol diluent, packaged in 0.25-mL plastic straws and slowly cooled to 5°C over a period of 1–2 h. Cooled straws are frozen by suspending within liquid nitrogen vapor above the liquid nitrogen surface before plunging into the liquid phase. Straws are thawed briefly in air before immersing in a 35°C water bath for 15 s, and often are used directly for insemination without any further processing.

Item Type:Book Section
Additional Information:Mammalian spermatozoa were among the very first cells to be successfully cryopreserved and over the last five decades the use of frozen-thawed semen for artificial insemination has come to play an important role in domestic livestock production. More recently, semen freezing has increasingly been utilized in the establishment of genetic resource banks for endangered species. Semen is collected, most commonly either by use of an artificial vagina or by electroejaculation of an anaesthetized animal, and basic sperm parameters assessed. Semen is extended using a TEST-egg yolk-glycerol diluent, packaged in 0.25-mL plastic straws and slowly cooled to 5°C over a period of 1–2 h. Cooled straws are frozen by suspending within liquid nitrogen vapor above the liquid nitrogen surface before plunging into the liquid phase. Straws are thawed briefly in air before immersing in a 35°C water bath for 15 s, and often are used directly for insemination without any further processing.
Keywords:Cryopreservation, Mammalian spermatozoa, Artificial insemination, Livestock - breeding
Subjects:C Biological Sciences > C142 Reproductive Biology
D Veterinary Sciences, Agriculture and related subjects > D421 Livestock Husbandry
C Biological Sciences > C110 Applied Biology
Divisions:College of Science > School of Life Sciences
ID Code:1300
Deposited By: Jill Partridge
Deposited On:08 Oct 2007
Last Modified:18 Jul 2011 16:18

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