Immobilization of hog pancreas lipase in macroporous poly(vinyl alcohol)-cryogel carrier for the biocatalysis in water-poor media

Plieva, F. M. and Kochetkov, K. A. and Singh, Ishwar and Parmar, V. S. and Belokon', Y. N. and Lozinsky, V. I. (2000) Immobilization of hog pancreas lipase in macroporous poly(vinyl alcohol)-cryogel carrier for the biocatalysis in water-poor media. Biotechnology Letters, 22 (7). pp. 551-554. ISSN 0141-5492

Full content URL: http://dx.doi.org/10.1023/A:1005660125927

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Item Type:Article
Item Status:Live Archive

Abstract

Hog pancreas lipase was covalently attached to the beads of poly(vinyl alcohol)-cryogel - a macroporous hydrogel prepared by means of freeze-thaw technique. The immobilized biocatalyst thus obtained was examined in the reaction of enantioselective hydrolysis of the ethyl ester of N-benzylidene derivative of DL-phehylalanine in the medium of acetonitrile (contained 5 vol.% of water without any buffers). Eighty-three %-enantiomeric excess of the L-amino acid was reached after 144 h. Virtually the same result was obtained in the repeated use of the same immobilized biocatalyst after its 6-months-storing in a refrigerator.

Keywords:immobilized lipas, poly(vinyl alcohol)-cryogel carrier}
Subjects:B Subjects allied to Medicine > B200 Pharmacology, Toxicology and Pharmacy
Divisions:College of Science > School of Pharmacy
ID Code:11316
Deposited On:23 Jul 2013 19:25

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